Roadmap for Phage technologies

What are we trying to achieve and why? What is the problem we are trying to solve?

Using phages as a delivery system for factors that modulate AMR by targeting AMR plasmids or targeting plasmid-mediated virulence factors.

What are the scientific and technological challenges (knowledge gaps needing to be addressed)?

AMR and virulence gene stability.
Better understanding of AMR and virulence plasmids and phages’ ability to selectively target plasmid-containing cells also needed.
One main problem is that phages may also increase horizontal gene transfer (they do so in their natural environments). Survey horizontal gene transfer (HGT).

What approaches could/should be taken to address the research question?

Targeting the sex pili to eliminate AMR transfer.

What else needs to be done before we can solve this need?

Existing knowledge including successes and failures