Projects
DEVELOPING CRISPR-BASED IN VITRO FUNCTIONAL GENOMIC SCREENING RESOURCES IN CHICKENS
Summary
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<b>Animal Health Component</b>
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<b>Research Effort Categories</b><br>
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Objectives & Deliverables
<b>Project Methods</b><br> We use the CRISPR/Cas9-mediated gene knock-in method to establish the cell lines that express CRISPRa or CRISPRi elements in five different chicken cell lines (DF1: embryonic fibroblast, DT40: B-cell, HD11: macrophage, LMH: liver epithelial, PGC: primordial germ cells). Once these cells are thoroughly characterized, we will test the CRISPR-based targeted gene modulation activity of the established cell lines by targeting the promoter and/or enhancer regions of candidate genes. Lastly, we will develop single-cell-based high-throughput CRISPR screening (scCRISPR-seq) using the developed cell lines and optimize the protocol as well as the bioinformatics pipeline.
Principle Investigator(s)
Planned Completion date: 31/03/2026
Effort: $650,000.00
Project Status
ACTIVE
Principal Investigator(s)
National Institute of Food and Agriculture
Researcher Organisations
PENNSYLVANIA STATE UNIVERSITY
United Kingdom