Influenza roadmap:
Diagnostic Tests
Roadmap for the development of diagnostic test for influenza
Download Influenza-diagnostic-development-roadmapA
Direct detection
Direct detection
Research Question
- Rapid direct detection of influenza viruses and its variants
Research Gaps and Challenges
- Cost-effective and rapid direct detection of replication-competent influenza virus
- Matrix-specific effects
- Overcoming embryonated eggs as the main propagation vehicle retaining sample authenticity (re sample passage in cells/eggs)
- Host antibodies that neutralize virus
- Field based diagnostic for rapid early assessment with perhaps lab based confirmation/subtyping
- Genetic changes associated with different systems
- Low viral load in the field samples
- Mixed subtypes in field samples (coinfections)
Solution Routes
- Improvement of virus isolation and propagation by new in vitro cell culture techniques
- Improvement of systems for viral replication/growth
- Build cell culture collections
- Modify existing cell lines to be more sensitive by expressing or repressing genes
- Pen-side tests?
Dependencies
- Availability of SPF eggs in low resource settings
- Cell culture collections
- Crispr/Cas technology with avian cell culture systems
- Germ line manipulation tools in avian species
- Sharing of resources
- Early assessment linked with automatic alerts to advisors/central labs for additional tests
Projects
What activities are planned or underway?
Development of Vaccines for HPAI in Cattle, Turkey, Pigs, and Small Ruminants and Food Safety Studies Related to HPAI Virus in Food Products
Planned Completion date 25/08/2029
Source Countries:
United States
Automated non-invasive Detection of Surface Contamination and Optimizing Surface Biofilm Removal Conditions in Large-Scale Food Processing Equipment
Planned Completion date 31/08/2028
Source Countries:
United States