Roadmap for the development of candidate vaccine for bTB

What are we trying to achieve and why? What is the problem we are trying to solve?

Identifying and removal of the factors contributing to pathological changes are essential for generating rationally
attenuated vaccines

What are the scientific and technological challenges
(knowledge gaps needing to be addressed)?

What approaches could/should be taken to address the research question?

Generation of a range of knock-out bacteria where putative virulence factors have been removed and their use in
experimental infections

What else needs to be done before we can solve this need?

Improved understanding of M bovis – macrophage interaction – M bovis and macrophage gene expression in different in vivo environments (macrophages from naïve and immune hosts)

Existing knowledge including successes and failures