Projects
PARTNERSHIP: NEXT GENERATION SURVEILLANCE FOR TICK-SWINE CONTACT IN ENDEMIC AND NON-ENDEMIC REGIONS FOR AFRICAN SWINE FEVER VIRUS
Topic: African Swine Fever
Summary
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<b>Animal Health Component</b>
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<b>Research Effort Categories</b><br>
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Objectives & Deliverables
<b>Project Methods</b><br> We will measure contact between ticks and swine with two approaches. One will be the bloodmeal metabarcoding which utilizes ticks captured in nature and a DNA-based PCR of conserved vertebrate genes, followed by amplicon deep sequencing and bioinformatics, will reveal the diverse vertebrate hosts that ticks are feeding on. The other tool is a serological biomarker which detects antibodies in swine that are developed in response to tick feeding, which introduces salivary proteins. These proteins that are specific to Ornithodoros soft tick species will be identified and generated as recombinant?proteins to be used in salivary bioassays of swine with known tick exposure (experiments conducted in Uganda) and swine of unknown tick exposure (swine serum banked in Uganda).
Principle Investigator(s)
Planned Completion date: 30/06/2028
Effort: $800,000.00
Project Status
ACTIVE
Principal Investigator(s)
National Institute of Food and Agriculture
Researcher Organisations
TEXAS A&M UNIVERSITY
United Kingdom